Supplementary Figure 6: Axial calibration of the photometric ratio and axial estimation error caused by small single-mode aberrations.
From: Impact of optical aberrations on axial position determination by photometry
(A) The photometric ratio over six 45-nm-bead measurements after aberration correction (Exp.) and simulated vectorial PSFs (Sim.) as a function of axial position (see Supplementary Methods). (B) The estimated axial position of aberration-corrected beads as a function of the calibrated axial position shown in (A). Around focus the error is a bit larger as expected owing to the near-parabolic shape of the photometric ratio curve (see Supplementary Methods for a description of the error analysis). (C) Estimated axial position for single-mode aberrations on 45-nm beads using the calibrated axial position from the aberration-corrected data in (A). The gray dashed line is used to guide the eye for the correct estimation. Experimental data (Exp.) in A,B,C are shown as mean ± s.d. over six bead measurements. (D) Error of the estimated axial position as the distance between the mean estimated position and the true (calibrated) position.
