Supplementary Figure 1: Orthogonal gene control with catalytically active Cas12a-VPR.
From: Multiplexed orthogonal genome editing and transcriptional activation by Cas12a
(a) Schematic depicting the strategy for Cas12a-VPR in which 20-nt guides produce indels and 15-nt guides allow transcriptional activation. (b) Overview of vector constructs. U6, Pol III promoter; NLS, nuclear localization signal;DR, direct repeat sequence; LTR, long terminal repeat; WPRE, woodchuck hepatitis virus post-transcriptional regulatory element;IR, inverted repeat;pA, polyA; PGK, phosphoglycerate kinase 1 promoter; puro = puromycin resistance; Blas = blasticidine resistance; EFs, truncated human elongation factor-1; EF1, human elongation factor-1 promoter; Mir30-shRNA-REN, microRNA-adapted short hairpin RNA targeting renilla; GFP, green fluorescent protein; BFP, blue fluorescent protein; VPR, herpes simplex virus-derived VP16 activator, human NF-KB p65 activation domain, Epstein–Barr-virus-derived R transactivator (Rta)).
