Supplementary Figure 3: Comparison of transcriptional-activation capabilities of AsCas12a-VPR with LbCas12a-VPR and deadLbCas12a-VPR.
From: Multiplexed orthogonal genome editing and transcriptional activation by Cas12a
a) Flow cytometry-based RFP quantification in murine reporter cell lines stably expressing either AsCas12a-VPR1 or LbCas12a-VPR and crRNAs harboring either the As-DR or Lb-DR. TRE-guide length was varied from 20-nt to 12-nt. ctrl = Rosa26-guide. Cells were cultivated off Dox. Mean ± s.d.; N = 3 independent experiments. b) Flow cytometry-based RFP quantification in murine reporter cell lines stably expressing deadLbCas12a-VPR. TRE-guide length was varied from 20-nt to 12-nt. crRNAs harboring the Lb-DR were used. ctrl = Rosa26-guide. Cells were cultivated off Dox. Mean ± s.d.; N = 3 independent experiments.
