Supplementary Figure 4: Comparison of transcriptional-activation capabilities of AsCas12a-VPR with other CRISPR effectors.
From: Multiplexed orthogonal genome editing and transcriptional activation by Cas12a
Flow cytometry-based RFP quantification in reporter cell lines stably expressing indicated CRISPR-effectors. A Doxycycline-inducible TRE-RFP-shp53 reporter was used to assess transcriptional activation. Mouse cells stably expressing indicated CRISPR-effectors were transduced with 15-nt or 20-nt TRE-guides to activate RFP. Cas12a and Cas9 guides were position matched and embedded in respective expression vectors. Mean ± s.d.; N = 3 independent experiments.
