Supplementary Figure 5: Transcriptional activation of endogenous genes with catalytically active AsCas12a-VPR and 15-nt guides, and comparison to LbCas12a-VPR or deadLbCas12a-VPR.
From: Multiplexed orthogonal genome editing and transcriptional activation by Cas12a
a) Relative mRNA expression after co-transfection of HEK293T cells with AsCas12a-VPR and 15-nt guides targeting the promoter region of indicated genes. N = 2 independent experiments. b) Relative mRNA expression after co-transfection of HEK293T cells with either AsCas12a-VPR, LbCas12a-VPR or deadLbCas12a-VPR and crRNAs. crRNAs were targeted to the promoter of TTN (left) or NPY1R (right). For TTN, N = 3 independent experiments. For NPY1R, N = 2 independent experiments.
