Supplementary Figure 7: FRC-based resolution scaling on ISM with increasing excitation power.
From: A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
(a) Series of tubulin (labeled with Abberior STAR red) images of the same area for ‘ideal’ confocal (top), ‘open’ confocal (middle) and APR-ISM (bottom) as a function of the excitation beam power. Bleaching was negligible across the whole imaging experiment. Pixel dwell time, 100 μs. Pixel size, 37.5 nm. Image format, 400 × 400 pixels. Excitation power Pexc: 50, 55, 62, 70, 90, 110, 170, 220, 250, 350, 520, 700, 890, 1,000, 1,300 and 2,000 nW. Scale bars, 1 μm. (b) Fourier-ring-correlation curves for different excitation beam powers and different imaging modalities: ideal confocal (top), open confocal (middle) and APR-ISM (bottom). The fixed 1/7 threshold value is also reported with the curves. Resolution (based on the FRC analysis and the 1/7 threshold value) as a function of excitation power for the three different imaging modalities is reported in Fig. 1f. Data are representative of n = 2 experiments.
