Supplementary Figure 8: Image scanning microscopy for live-cell mitochondria imaging.
From: A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
(a) Side-by-side comparison of ‘ideal’ confocal (top), ‘open’ confocal (middle-top), APR-ISM (middle-bottom) and deconvolved ISM++ time-lapse (3 min, 24 frames) of mitochondria labeled with MitoTracker Deep Red in a live cell. For each imaging modality, three representative frames are shown (0 s, 90 s and 180 s). Pixel dwell time, 30 μs. Pixel size, 40 nm. Image format, 500 × 500 pixels. Excitation power Pexc, 140 nW. Scale bars, 1 μm. (b) Maximum intensity projections (color-coded by time) of the time-lapses for the different imaging modalities. These projections allow one to identify the fraction of mitochondria with minimal mobility (white) from the mobile one (colored). Data are representative of n = 10 experiments.
