Supplementary Figure 12: Fluorescence lifetime image scanning microscopy of membrane labeled with ANEP. | Nature Methods

Supplementary Figure 12: Fluorescence lifetime image scanning microscopy of membrane labeled with ANEP.

From: A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM

Supplementary Figure 12

Side-by-side comparison of the fitting-based FLIM (left; 0.25 AU) and FLISM (right) images of membrane labeled with ANEP. Purple pixels correspond to out-of-range fluorescence lifetime values, which probably resulted from artifacts during the time-domain (fitting) analysis. All pixels were analyzed. Pixel dwell time, 300 μs. Pixel size, 62.6 nm. Image format, 800 × 800 pixels. Excitation power Pexc, 3 μW. Scale bars, 1 μm. Data are representative of n = 4 experiments.

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