Supplementary Figure 15: Image scanning microscopy combined with fast resonant scanning.
From: A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
Side-by-side comparison between ‘ideal’ confocal, ‘open’ confocal and APR-ISM images of tubulin stained with Alexa Fluor 546 (format, 256 × 256 pixels; resonant frequency, 7.9 kHz; zoom factor, 8 × , which results in a pixel size of 103 nm and a minimum pixel dwell time of about 70 ns; 64 line integrations). Insets show magnified views of the regions outlined by white boxes. Scale bars, 1 μm. Data are representative of n = 10 experiments.
