Supplementary Figure 3: Image scanning microscopy with adaptive (A) pixel reassignment (PR).
From: A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
(a) Side-by-side comparison of the effective PSFs for ‘ideal’ confocal (0.2 AU), ‘open’ confocal (1.4 AU), uncorrected ISM (the pixel- reassignment method uses theoretical shift vectors), and ISM (the pixel-reassignment method uses estimated shift vectors). Scale bar, 500 nm. (b) Fingerprint maps superimposed with the shift vectors for the uncorrected ISM (left) and the true ISM (right). Scale bars, 50 nm. (c) Radial PSFs obtained from the intensity profiles of (a). The top panel shows the un-normalized PSFs, and the middle and bottom panels show the normalized PSFs, together with the Gaussian fitted data. Pixel-dwell time, 50 μs. Pixel-size, 5 nm. Image format, 400 × 400 pixels. Scale bars, 500 nm.
