Supplementary Figure 6: Image scanning microscopy of fluorescent beads.
From: A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
(a) Side-by-side comparison of ‘ideal’ confocal, ‘open’ confocal, APR-ISM and deconvolved ISM++ (ten iterations) images (500 × 500 pixels, 40-nm pixel size) of 20-nm red fluorescent beads. Pixel dwell time, 50 μs. Pixel size, 40 nm. Image format, 500 × 500 pixels. Excitation power Pexc, 56 nW. Scale bars, 1 μm. (b) Magnified views of the regions outlined by white boxes in (a). Scale bars, 1 μm. (c) Line intensity profiles across two close fluorescent beads at the position of the arrowheads in (b) for the different imaging modalities. Data are representative of n = 5 experiments.
