Supplementary Figure 8: Target cell trogocytosis resolves cognate antigen-expressing target cells from noncognate antigen-expressing cells.

(a) Schematic of experiment. (b) Representative flow cytometry plots for a 1:1 mixture of NYESO1-K562 and MART1-K562 cells co-incubated with either 1G4-Jurkat (top) or F5-Jurkat (bottom) cells (10:1:1 Jurkat: NYESO1-K562:MART1-K562). The trogocytosis⁺ and trogocytosis^– populations were verified for antigen specificity by use of either F5 TCR or 1G4 TCR dextramer staining. Data are representative of two independent experiments. (c) Quantification of antigen-specific target cell in trogocytosis⁺ and trogocytosis^– populations via F5 TCR or 1G4 TCR dextramer staining (n = 3). Statistical analysis of quantification was performed using unpaired two-tailed Student’s t-test. Data in b, c are presented as mean ± s.e.m. and are representative of two independent experiments.