Supplementary Figure 2: Target cell trogocytosis occurs in an antigen-specific manner. | Nature Methods

Supplementary Figure 2: Target cell trogocytosis occurs in an antigen-specific manner.

From: T cell antigen discovery via trogocytosis

Supplementary Figure 2

(a) ZsGreen cannot be transferred from K562 cells to Jurkat cells. Representative flow cytometry plot of Zsgreen level in F5-CD8-Jurkat or 1G4-CD8-Jurkat following co-incubation with MART1-K562. (b,c)The kinetics of trogocytosis for TCR–cognate pMHC interaction. Co-incubation of 1G4-CD8-Jurkat cells with NYESO1-K562 or F5-CD8-Jurkat cells with MART1-K562 for indicated time and trogocytosis was assessed using anti-muTCR antibody (n = 3). Data are presented as mean ± s.e.m. (d) Immunofluorescence staining of co-incubated F5-CD8-Jurkat with NYESO-K562 (Zsgreen+) or MART1-K562 (Zsgreen+) by MART1 pMHC dextramer (yellow). Data in this figure are representative of at least two independent experiments.

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