Supplementary Figure 3: Trogocytosis can be tracked by multiple protein transfer. | Nature Methods

Supplementary Figure 3: Trogocytosis can be tracked by multiple protein transfer.

From: T cell antigen discovery via trogocytosis

Supplementary Figure 3

(a) Antigen-specific transfer of the T cell membrane proteins, CD3 and CD8, from CD8-expressing F5-Jurkat and 1G4-Jurkat cells to K562 cells (ZsGreen+), as assessed by anti-CD3 and anti-CD8 antibodies (5:1 J:K). (b) Antigen-specific transfer of the K562 cell membrane protein, HLA-A2, from K562 cells to Jurkat cells (ZsGreen–), as assessed by an anti-HLA-A2 antibody (5:1 J:K). (c, d) Concomitant reduction of membrane proteins from donor cells. Data are representative of three independent experiments.

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