Supplementary Figure 9: Illustration of mass distribution correction in late mitosis.
From: Noninvasive monitoring of single-cell mechanics by acoustic scattering
a, DIC images of the mitotic L1210 cell acquired on-chip, simultaneously with SNACS and buoyant mass measurement in late mitosis. For each time point, cells were fitted with overlapping spheres (yellow dotted lines) based on the measured elongation length (blue double arrows). Each number on the image marks the time progression. Scale bars, 10 μm. b, Cell length measured (blue dots) is plotted as a function of time after onset of anaphase. Numbers correspond to the images shown in a. The linear elongation derived from the first (1) and last image (12) is shown by the red line. c, SNACS before correction (gray), after correction for the mass elongation using length directly measured from DIC images (blue dots) and assuming linear elongation (red), plotted as a function of time after onset of anaphase. Numbers correspond to the images in a. See Supplementary Note 3 for additional details.
