Supplementary Figure 10: SNACS change occurs in early mitosis and is reduced by EIPA treatment.

a, Buoyant mass (black) and SNACS (red) of L1210 treated with 2 μM RO-3306, a CDK1 inhibitor. Arrowhead marks the start of drug exposure. The cell arrested in G2 continues to grow above the average size of L1210 cells at the mitotic entry (gray dashed line). b,c, Buoyant mass (black), SNACS (red) and FUCCI (green, mAG-hGem) of a control L1210 cell (b) and an L1210 cell treated with 10 µM ethylisopropylamiloride (EIPA, an inhibitor of Na⁺/H⁺ antiporters) (c). The fluorescence detection limit of our system is shown by a green band at the bottom. An abrupt decrease in FUCCI (degradation of Geminin) marks the metaphase–anaphase transition. Vertical lines separate the cell cycle positions marked by color bars as shown in Fig. 3c. P, prophase; M, metaphase; A, anaphase; T, telophase.