Supplementary Figure 15: Orientation-dependent noise and mass distribution correction during late mitosis.
From: Noninvasive monitoring of single-cell mechanics by acoustic scattering
a, Top, schematic showing the two extreme orientations of a cell during late mitosis: cells flowing parallel to the channel (θ = 0) and maximally rotated (θ = θmax). Note that the dimension of the channel walls (black lines) and elongation length of a cell at a given time point determines the maximal rotation angle (θmax). Bottom, SNACS is corrected assuming three different orientations relative to the channel when cell is at the node: θ = 0 (blue dash), θ = θmax (red dash) and θ = θmax/2 (black solid). The gray area marks the orientation-dependent noise. See Supplementary Fig. 9 for raw SNACS. b, Effect of uncertainty in cell orientation on measured SNACS and corrected SNACS for two hypothetical cells both elongating as L1210 cells in mitosis (top panel, blue), but with different mechanical changes (top panel, red): zero change (left) and dynamic mechanical change similar to L1210 cells in mitosis (right). Two extreme orientations illustrated in a mark the boundary (blue and red dash) of orientation-dependent noise (gray area) in measured SNACS (middle panel). Corrected SNACS signals assuming an intermediate orientation (θ = θmax/2) during the entire time course are shown in the bottom panel. Representation of SNACS traces corresponding to each orientation are the same as in a. See Supplementary Note 4 for additional details.
