Supplementary Figure 4: Illustration of the methodology used for obtaining the size-normalized acoustic scattering (SNACS) from the node deviation.
From: Noninvasive monitoring of single-cell mechanics by acoustic scattering
a,b, Steps for obtaining SNACS: The resonant frequency shifts (Δf/f) for each cell (1–4; colored curves) are used to measure the buoyant mass (BM) at the antinode and the node deviation at the node (a). Scatter plot of volume-normalized node deviation (NV) versus volume (V) (b). The volume of each cell (V1,2,3,4) is obtained from the corresponding buoyant mass (BM1,2,3,4) using the included equation. \(\overline {\Delta \rho }\) is the median density of the population relative to the fluid. Data points of cells (1–4) are fit with isoelasticity lines (colored lines) with slope m (c). We obtained the slope (m) by performing a linear regression on the population data in the NV-versus-V scatter plot shown in Fig. 2b. The SNACS of each cell is obtained via the included equation (d). Graphically, the SNACS of each cell corresponds to an NV value at the intersection point between the isoelasticity line (colored lines) and the vertical line, V = Vref (black dotted line), where Vref is set as the median volume of the population. See Methods for additional details.
