Supplementary Figure 14: The 13CF TROSY resonance is the slowest relaxing nucleus in biological NMR. | Nature Methods

Supplementary Figure 14: The 13CF TROSY resonance is the slowest relaxing nucleus in biological NMR.

From: Aromatic 19F-13C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics

Supplementary Figure 14

a,b, Theoretical transverse relaxation rates of 13CF, 15NH and 13CH at the magnetic field strength where the TROSY effect for any given spin-pair is maximized, calculated for (a) a 42-kDa protein (τc at 25 °C = 25 ns) and (b) a 110-kDa protein (τc at 25 °C = 65 ns). Simulated free induction decays (top) compare and visualize the influence of transverse relaxation on the height and line width of the TROSY resonance of the aromatic 13CF, aromatic 13CH and backbone 15NH spin states.

Back to article page