Supplementary Figure 2: Selective detection of the TROSY component. | Nature Methods

Supplementary Figure 2: Selective detection of the TROSY component.

From: Aromatic 19F-13C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics

Supplementary Figure 2

a,b, 13C-19F HSQCs of 3-19F13C Tyr-labeled MBP recorded at 600 MHz: (a) decoupled and (b) coupled. The expected narrowing for the TROSY component is observed in b. A dashed rectangle highlights the four components of one decoupled spin-pair (red). The top right component is clearly more intense than the top left component and the bottom components, which are anti-TROSY with respect to 13CF are substantially broadened. In addition, two intense (more mobile) 13CF TROSY peaks are highlighted and a 1D trace identifies the component to the right (top right) as the TROSY component. c, The TROSY component was selectively observed by the ST2PT scheme. The negative signal (purple) originates from imperfect cancelation of the anti-TROSY component.

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