Supplementary Figure 8: Dominant negative regions in ribosomal proteins.
From: High-throughput identification of dominant negative polypeptides in yeast
Ribosomal proteins were mapped onto the cryo-EM structure of the translating ribosome (PDB 4V6I), with the small subunit (40S) shown in gray, the large subunit (60S) in darker gray, the 18S and 25S rRNAs in gold, the P-site tRNA in blue, and proteins with dominant negative polypeptides in red. Around the structure are depletion plots that show domains in these proteins with overlapping dominant negative polypeptides. The y-axis of each depletion plot shows the coverage of overlapping fragments for in-frame depleted (red), in-frame neutral (pink), out-of-frame depleted (dark gray) and total fragments in all frames (light gray) (Methods, Supplementary Data 1).
