Supplementary Figure 10: Deleterious effects of dominant negative polypeptides are exacerbated in media in which the target gene is essential.

a, Coverage charts from the cDNA library selection in minimal media are shown for three genes related to amino acid metabolism. Our analysis identified each of these genes as having dominant negative regions. A representative fragment (indicated by red rectangles below coverage charts) was selected for each gene, and the polypeptide fragment was overexpressed in yeast cells. b–d, Bar plots showing growth rates of cells containing each dominant negative fragment grown in three types of media, each lacking an amino acid related to the expected function of the target protein: (b) threonine; (c) adenine; and (d) phenylalanine. The leftmost pair of bars corresponds to the protein fragment whose function corresponds to the amino acid left out of the media (e.g., a fragment from Thr1 is the first bar shown in b). For each media type, fragments not expected to affect growth were tested alongside an empty vector as control. N = 3 for all strains; bar height corresponds to mean, and error bars correspond to s.e.m. Significance from two-sided t-test indicated by asterisks (Thr1 P = 0.038, Ade3 P = 0.0052, Aro1 P = 0.073; ns, not significant).