Supplementary Figure 1: Double knockout of Nf1 and Pten by a single Cpf1 crRNA array.
From: In vivo profiling of metastatic double knockouts through CRISPR–Cpf1 screens
a, Schematic maps of the constructs for double knockout (DKO) experiments using a single Cpf1 crRNA array. b, One-step cloning strategy for DKO experiments using CRISPR–Cpf1. c, Schematic of the experimental approach for evaluating whether both permutations of an Nf1 and Pten dual-crRNA array can induce mutagenesis at both target loci. d, Seven days after lentiviral infection, genomic DNA was collected for mutation analysis by Nextera sequencing. For each treatment condition, mutations were identified at the genomic loci targeted by crPten (left column, blue) and by crNf1 (right column, orange). Variant frequencies associated with each mutation are shown in the boxes to the right; for each condition, the top five most frequent variants are shown. The total mutation frequencies are noted below.
