Supplementary Fig. 2: Sample stage and mounting.
From: Instantaneous isotropic volumetric imaging of fast biological processes
(a) Close-up of the composite stage (left panel). Fine positioning of the sample is achieved with a combination of coarse manual XYZ positioning, fast piezo stack positioning for light-sheet acquisition or changing the volumetric field-of-view in Iso-LFM, as well as a miniature rotation stage. (b) Medaka fish hatchlings are first mounted in an agarose filled capillary (inner diameter 1.6 mm) and thereafter the agarose tube is attached to a custom sample mount which is positioned into the focal volume of excitation and both detection objectives. (c) Close-up of the sample stage. Note that only one detection objective is shown here for clarity (i.e. View 2 has been removed). Illumination (detection) objective is labelled by a cyan (green) arrow.
