Supplementary Fig. 5: Overlap of light-sheet and Iso-LFM volumes. | Nature Methods

Supplementary Fig. 5: Overlap of light-sheet and Iso-LFM volumes.

From: Instantaneous isotropic volumetric imaging of fast biological processes

Supplementary Fig. 5

Lateral (top) as well as axial (bottom) maximum intensity projections of fluorescent beads distributed in 3D in agarose, imaged by either light sheet (cyan) or Iso-LFM (red) and a 40x 0.8NA objective. The mean distance in 3D space between light-sheet and Iso-LFM reconstructions of the same beads is ~1.5 ± 0.7 µm (mean ± SD, n = 24 beads) and therefore smaller than the resolution of Iso-LFM. Scale bar is 10 µm.

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