Supplementary Fig. 6: Characterization of performance for 22.5x 0.5NA objectives.
From: Instantaneous isotropic volumetric imaging of fast biological processes
(a,b) Maximum intensity projections of reconstructed fluorescent beads and their respective resolution (FWHM) for single-view and dual-view, respectively. The lower panels depict the measured PSF at −80 µm off the focal plane (averaged over n = 12 beads) with lateral and axial FWHM indicated. Scalebars upper panel 10 µm and lower left panel 5 µm. (c) Mean values of lateral and axial PSF FWHM for the single-view (n = 2192 beads total) and the dual-view (n = 2816 beads total) case across imaging volume (steps of 30 µm). Multiview deconvolution improves axial resolution across the entire overlapping volume and achieves near-isotropic resolution of better than ~5 µm (5.2 ± 1.3 µm, ~2.7 ± 0.5 µm and ~4.8 ± 1.2 µm in x/y and z, respectively). Centre lines represent mean and shadings denote SD.
