Supplementary Fig. 7: Blood flow quantifications from single-cell tracking using Iso-LFM.
From: Instantaneous isotropic volumetric imaging of fast biological processes
Single blood cells were manually tracked in 3D using the Fiji plugin MtrackJ1. Mean velocity (solid line) of all tracked blood cells (n = 28) over time. Shaded region denotes SD. Green and red dots denote velocities of tracked cells in the center and close to the vessel boundary, respectively, as indicated in the inset (scale bar 20 µm). On average, cells in the center of the vessel show higher velocities compared to those close to the vessel’s wall.
