Supplementary Figure 7: High-resolution imaging through layer 5b vS1 cortex in a Rbp4-cre mouse labeled by mRuby2.
From: Direct wavefront sensing enables functional imaging of infragranular axons and spines
(a) The three-dimensional image stack from in vivo imaging of mRuby2 labeled layer 5 pyramid neurons in a 150×150×810 µm3 volume of vS1 cortex at an excitation wavelength of λ = 1.07 µm. Full AO corrections were measured and applied every 50 µm of depth. An enlarged view of Fig. 2e. (b) Images at different depths, from 560 to 765 µm below the pia, with System AO and Full AO corrections. The corresponding correcting wavefront is shown.
