Supplementary Figure 8: Adaptive optics correction over wide field at a depth of 570 µm below pia.
From: Direct wavefront sensing enables functional imaging of infragranular axons and spines
The subject is a Thy1-YFP mouse. (a, b) Images tiled by 3×3 sub-region scanning with System AO correction and Full AO correction are compared. (c) Zoom-in view of the boxed region of the image in a to compare the images with System and Full correction at different regions over a wide field. (d) Signal profiles of the white lines in a are compared between System and Full AO as a representative of 3 measurements in 2 mice. (e) Scanning path for wide field AO imaging with corresponding subregion wavefronts as shown in f, which were sequentially applied onto the DM and synchronized to sub-region switching by internal triggers. (f) Full AO correcting wavefronts used in panel b.
