Supplementary Figure 10: Full AO correction enables imaging of Cy5.5-dextran labeled vascular and red blood cell flux through the polished and reinforced thinned-skull window at depth in vS1 cortex.
From: Direct wavefront sensing enables functional imaging of infragranular axons and spines
(a) Maximum intensity projections on y axis of the 140 × 140 × 600 µm3 volume images are compared between System (left) and Full (right) AO correction. Wavefronts at four different depths are shown on the right. Experiments were repeated in 2 mice with 2 measurements across depths. (b) Lateral images at 225, 325, 425 and 525 µm depth belong to the volume shown in panel a are compared between System (left) and Full AO (right) correction. (c) Signal profiles along the yellow dashed line in panel b are compared between System (blue) and Full AO (red) correction. (d) Imaging of vascular labeled by Cy5.5-dextran at 383 µm depth through the thinned-skull window with Full AO correction. (e) Red blood cell flux shown as line scan signals along the red and green lines in panel d are compared between System and Full AO. (f, g) Same imaging as panels d and e but at a depth of 541 µm below the pia. (h) Dependence of signal enhancement of red blood cell flux by AO correction as a function of the depth below the pia. Signal enhancement is defined as the ratio of peak-to-valley contrast of the line scan image of Full AO to System AO. 60 measurements over 3 mice were computed and shown in the boxplot with maximum, minimum, third quartile, first quartile and median (n ≥ 6).
