Supplementary Figure 11: Wide-field imaging through thinned-skull window with Full AO and signal enhancement analysis.
From: Direct wavefront sensing enables functional imaging of infragranular axons and spines
(a,b) Neuronal images at 320 µm below the pia in S1 cortex of Thy1-YFP mouse with System AO and Full AO correction and corresponding k-space map shown in panel c. (d, e) Vascular images at the same region of panel a with System AO and Full AO corrections. (f) Tiled scanning path is used for wide field AO imaging, same as in Supplementary Fig. 8. (g) Wavefronts used for correction in panels b and e. (h) Signal profiles along the dashed lines in panels a and b respectively, are compared between System AO (blue trace) and Full AO (red trace) correction. (i) Signal enhancement was calculated by the ratio of peak-to-valley contrast of the line signal profiles across the fine process in the neuronal images in vS1 between Full AO and System AO. 128 measurements in 3 Thy1-YFP or Thy1-jRGECO1a mice were shown as the boxplot with maximum, minimum, third quartile, first quartile and median (n ≥ 10).
