Supplementary Figure 13: Assessing phototoxicity by measuring sensory evoked glutamate response of thalamocortical axons in vS1 L5 during chronic imaging with adaptive optics.
From: Direct wavefront sensing enables functional imaging of infragranular axons and spines
(a) Experiment of 30-minutes continuous imaging with rhythmic air puff whisker stimulation. (b) The experiment as in panel a was repeated at same location for 3 days. Images of the thalamocortical axons labeled by SF-Venus-iGluSnFR.A184S at the same region of B1 barrel at the depth of 660 µm and corresponding wavefronts for each day are shown. (c) Sensory evoked glutamate signals of the axons in the imaging field were averaged every 5 minutes (n = 15 trials) and shown during 30-minutes continuous imaging for each day. (d) Averaged glutamate baseline signal of 3 days based on 3 measurements.
