Supplementary Figure 14: Assessing phototoxicity by measuring sensory evoked calcium response of basal dendrites and spines in vS1 L5 during long-term imaging with adaptive optics.
From: Direct wavefront sensing enables functional imaging of infragranular axons and spines
(a, b) Same experiment as in Supplementary Fig. 13a was performed. Images of the dendrites and spines labeled by jRGECO1a in C1 barrel region at 650-µm depth. (c) Sensory evoked calcium signals of the dendrites in ROI7 and spines in ROI1 and ROI3 shown in a and b were averaged every 5 minutes during 30-minutes imaging session (15 trials). (d, e) Normalized amplitudes of sensory evoked calcium signals of 6 spines in ROI1-ROI6 as in panel b and 3 dendrites in ROI7-ROI9 as in panel a. Slopes were calculated by linear regression fit with initial point constraints for each ROI datasets. The standard deviation and the mean of the slope values were calculated and shown in panel d (n = 6) and panel e (n = 3). The black lines indicate the fitting curve using mean slope with constraint initial point.
