Supplementary Figure 2: Schematic and calibration of adaptive optical two-photon microscope setup.
From: Direct wavefront sensing enables functional imaging of infragranular axons and spines
(a) A tunable femtosecond beam is expanded and incident on the deformable mirror (DM). The DM, the X and Y galvo mirrors, and the back focal plane of the objective are all conjugated by the 4-f lens pair between each other. The Shack-Hartmann wavefront sensor (SHWS) is placed in a descan mode. The microlens array of the SHWS is further conjugated with the objective back focal place. During wavefront sensing, the fluorescence emission light is descanned and separated with the guidestar excitation beam by dichroic D4 before arriving at the microlens array of the SHWS. Function signals are collected onto multi-pixel photon counters. (b, c) System AO aberration calibration. Top panels show the changes of Zernike mode coefficients as a function of optimization cycles. Bottom panels show the system aberration represented by Zernike mode index at wavelengths of 1.07 µm and 1.25 µm after 30 cycles optimization. (d) Lateral and axial images and signal profiles of a 200-nm diameter fluorescent bead imaged at 1070-nm wavelength.
