Supplementary Figure 4: Wavefront sensing at depths up to 850 µm in living mouse cortex.
From: Direct wavefront sensing enables functional imaging of infragranular axons and spines
The signal-to-background ratio (SBR) of the spots image acquired by Shack-Hartmann wavefront sensor decays as the depth increases as a result of tissue scattering. (a) Maximum intensity projections along x axis (Δx = 200 µm) of the cortical vasculature labeled by Cy5.5-dextran. (b) Guide-star microvascular images acquired by two-photon imaging at 14 µm to 850 µm below the pia and their corresponding spot images acquired by the SHWS during direct wavefront sensing. (c) Histograms of the SBR at different depths for the data on panel b with enlarged histograms of the SBR≤1.1 shown as subgraphs from 500-µm to 850-µm depth. (d) Examples of the wavefront image spots with the SBR below the value 1.1. (e) Weight distribution as a function of SBR that is used for wavefront reconstruction.
