Supplementary Figure 6: Chromosome-Conformation-Capture-Carbon-Copy (5C) global heatmaps at the larger Zfp462 and Klf4 gene loci in LADL-engineered mouse embryonic stem (ES) cells compared to relevant controls.
From: LADL: light-activated dynamic looping for endogenous gene expression control
Heatmaps represent 5C signals in relative interaction frequency for an 804 kb genomic region around the Klf4 and Zfp462 genes (chr4:54,799,136–55,603,136) from mouse ES cells co-transfected with LADL (Anchor + Bridge + Target) constructs after 24 h blue light illumination at 5 mW/cm2 intensity (left column) and compared with two relevant controls: LADL–engineered cells in dark (middle column) and Empty target control in dark (right column). Zoom regions for engineered de novo loop (Box 1) and Klf4-stretch enhancer (SE) loop (Box 2) are shown in green boxes.
