Supplementary Figure 4: Characterization of H1, RUES2 and HYS0103 cells exposed to a localized source of 50ng/ml BMP4

(a) Representative fluorescence pictures for indicated markers in H1 cells after 48 hours of exposure to a localized 40ng/ml source of BMP4 in the microfluidic device. This experiment was repeated independently two times (with four samples per repetition) and showed similar results. (b) Fluorescence pictures for indicated markers in RUES2 cells after 48 hours of exposure to a localized 80ng/ml source of BMP4 in the microfluidic device. This experiment was repeated independently two times and showed similar results. (c) Mean (line) and standard error of the mean (light-colored area) of normalized fluorescence intensity for the indicated markers along the source-sink axis of multiple cell chambers of RUES2 cells after 48 hours of exposure to a localized 80ng/ml BMP4 source (n=7 independent samples). (d) Fluorescence pictures for indicated markers in HYS0103 hiPSC cells after 48 hours of exposure to a localized 20ng/ml source of BMP4 in the microfluidic device. This experiment was repeated independently two times and showed similar results. (e) Mean (line) and standard error of the mean (light-colored area) of normalized fluorescence intensity for the indicated markers along the source-sink axis of multiple cell chambers of HYS0103 hiPSC cells after 48 hours of exposure to a localized 20ng/ml BMP4 source (n=7 independent samples). Scale bar 200μm.