Supplementary Figure 8: Effect of counteracting BMP4 and NOGGIN sources on the patterning of HES3 MIXL1^GFP/+ cells.

(a) Boxplots of the distribution of HES3 MIXL1^GFP/+ cells positive for the reported markers along the axis of the cell chamber after 48 hours of exposure to counteracting sources of 50ng/ml BMP4 and 200ng/ml NOGGIN (n=8 independent samples). In the boxplots the center line represents the median, the box limits represent the first and third quartiles, the whiskers represent the upper and lower extremes of the distribution, while white circles represent outliers (defined as a data point located more than 1.5 times outside the interquartile range above the upper quartile or below the lower quartile). Single data points are also shown in red. (b) Combined graph of the quantification of MIXL1, T, SOX17 and CDX2 for of HES3 MIXL1^GFP/+ cells after 48 hours of exposure to counteracting sources of 50ng/ml BMP4 and 200ng/ml NOGGIN (from Fig. 6d). (c) Scheme that approximates the distribution of markers in a colony of HES3 MIXL1^GFP/+ cells after 48 hours of exposure to counteracting sources of 50ng/ml BMP4 and 200ng/ml NOGGIN.