Supplementary Figure 10: Free Ub staining by HA-tUI shows a different subcellular distribution than K48-linked polyUb or total conjugated Ub.
From: High-affinity free ubiquitin sensors for quantifying ubiquitin homeostasis and deubiquitination
HeLa cells were fixed with 4% PFA and stained with DAPI (blue), anti-K48 polyUb antibody (green), and HA-tUI (magenta) in panel a, or DAPI (blue), anti-Ub FK2 antibody (green), and HA-tUI (magenta) in panel b. For each cell, the images are from a single z-stack plane that shows both nucleus and cytoplasm. The cells shown are representative of more than 10 cells analyzed on each of two coverslips.
