Supplementary Figure 11: Competition binding assays to measure tUI affinity for Ub.
From: High-affinity free ubiquitin sensors for quantifying ubiquitin homeostasis and deubiquitination
Competition binding assays were performed with a, 70 pM Atto532-tUI or b, 50 pM Atto532-tUI in the presence of 80 pM Ub. tUI was titrated from 0.063 nM to 32 nM, from which a mean Ki of 194 pM was determined from fittings to the two independent data sets.
