Supplementary Figure 14: Neuronal morphology and response tuning before and 6 days after SLAP imaging.

An example L2/3 cortical neuron was imaged with SLAP for 64 trials at an average power of 87 mW on Day 1. Neuronal morphology (top) was imaged before SLAP recording (left) and in a second session 6 days later (right). Raster recordings of tuning (bottom) were interleaved with SLAP imaging on Day 1, and again 6 days later. We observed no signs of damage from SLAP imaging. Experiment was performed once.