Supplementary Figure 15: Characterization of RhoVR.Pip.Sulf 1.

(a) DIC image of cells loaded with RhoVR 1. (b) Fluorescence image of cells loaded with RhoVR 1. (c) DIC image of cells loaded with RhoVR.Pip.Sulf. (d) Fluorescence image of cells loaded with RhoVR.Pip.Sulf. Scale bar is 20 µm. (e) Quantification of relative RhoVR fluorescence in HEK cells. Bars denote mean ± S.D. for n = 4 (RhoVR 1) or 6 (RhoVR.Pip.Sulf) cells. (f) The fractional change in fluorescence is plotted vs. time for 100 ms hyper- and depolarizing steps (±100 mV, 20 mV increments) from a holding potential of -60 mV for a single HEK cells under whole-cell voltage-clamp mode. (g) A plot of % ΔF/F vs. final membrane potential (mV) summarizing data from 5 separate cells.