Supplementary Figure 8: Comparison of the dTAG system and the improved AID system for depleting endogenous LMNA.
From: An efficient auxin-inducible degron system with low basal degradation in human cells
(a) Scheme for tagging endogenous LMNA in A431 and HEK293A cells using CRISPR/Cas9-mediated HDR. A coselection strategy was employed for one-step generation of cells with both miniIAA7-mEGFP tagged LMNA and AtAFB2-mCherry-weak NLS. (b) Western blot analysis of endogenously tagged LMNA depleted with the dTAG or the improved AID system in the indicated cell pools. All immunoblots are representative of two independent experiments; uncropped scans of immunoblots are shown in Supplementary Fig. 10.
