Supplementary Figure 9: Reversibility in the improved AID system compared to the OsTIR1-miniIAA7 system or the dTAG system.
From: An efficient auxin-inducible degron system with low basal degradation in human cells
(a-b) Left: FACS plots showing GFP histograms before and after IAA washout with the indicated miniIAA7-mEGFP tagged proteins in A549 or A431 cells. Right: mean GFP intensities analyzed by FACS. Bars represent mean ± s.d; n=3 independent experiments. (c) Western blot analysis of endogenously tagged LMNA before and after washout with the dTAG system or the improved AID system in HEK293A cell pools. All immunoblots are representative of two independent experiments; uncropped scans of immunoblots are shown in Supplementary Fig. 10. (d) Schematic illustration of dTAG-13 bifunctional binding and IAA coreceptor binding.
