Supplementary Figure 7: Testing of the dTAG system for BRD4 degradation.
From: An efficient auxin-inducible degron system with low basal degradation in human cells
(a) Principle of protein depletion with the dTAG system. CRBN is an endogenous component in this system. (b) Scheme for tagging endogenous BRD4 in A431 and HEK293A cells using CRISPR/Cas9-mediated Precise Integration into Target Chromosome (PITCh). (c-d) Western blot analysis of dTAG-13 induced depletion of endogenously tagged BRD4 (c) and dTAG-13 washout (d) in the indicated cell pools. All immunoblots are representative of two independent experiments; uncropped scans of immunoblots are shown in Supplementary Fig. 10.
