Supplementary Figure 10: Design and measurement results of DNA origami structures.

(a) AFM image of the synthesized DNA origami. (b) Design of the DNA origami structures for DNA-PAINT imaging. (c and e) Averaged image of the 20 nm DNA origami structures reconstructed by centroid fitting (c) and ROSE (e), respectively, with the center of each cluster (red cross) compared with the designed lattice pattern (green cross). (d and f) Measured 20 nm structure lattice constants of c and e, shown as design schematics. (g) Averaged image of the 5 nm DNA origami structures reconstructed by ROSE, with the center of each cluster (red cross) compared with the designed lattice pattern (green pattern). (h) Multi 2D Gaussian fitting result of g, with the designed lattice pattern (green cross). (i) Measured 5 nm structure lattice constants of g, shown as design schematics. Scale bars: 100 nm in a, 20 nm in c, e, g and h. For the data in a, 5 experiments were repeated independently with similar results. For the data in c to f, the total number of averaged structures was 92, and the center indicates the mean value. 5 experiments were repeated independently with similar results. For the data in g to i, the total number of averaged structures was 169 and the center indicates the mean value. 5 experiments were repeated independently with similar results.