Supplementary Figure 13: Comparison of centroid fitting and ROSE imaging of clathrin-coated pits (CCP) in COS-7 cells.
From: Molecular resolution imaging by repetitive optical selective exposure
(a) Wide field of the fluorescent image. (b) Reconstructed image with ROSE, showing the hollow structures of the CCPs. (c and d) Zoomed in images of the reconstruction with conventional centroid fitting (c) and ROSE (d), respectively. (e) Distribution of the photon number of single molecules showing a mean number of 451 and median number of 391. (f) Intensity profile of the cross-section indicated in c and d. The diameter of the hollow structure was 90.3 nm, which could not be clearly resolved in (c) by the centroid fitting method. (g) Intensity profile of the cross section indicated in c and d. Scale bars: 1 μm in a and b; 500 nm in d. The width of ROI in c and d is 30 nm. For data in a to d, 5 experiments were repeated independently with similar results.
