Supplementary Figure 4: The relation between on-off switching times and the localization precision under various scan cycle times.

The simulated off-time subjects to exponential distribution with average values of 1 ms, 5 ms, 15 ms, 50 ms, 150 ms, 500 ms, 1,500 ms and 5,000 ms, and the simulated average on-time subjects to exponential distribution with average values from 1 ms to 100 ms, which covers most experimental conditions. The scan cycle time of 0.125 ms, 2.5 ms and 25 ms were tested, which represents the typical scan cycle time for resonant scanner, standard galvo scanner and sequential exposure to get 6 subimages without scanning configuration, respectively. For each condition, 1000 frames were generated for the statistical analysis, each point represents the mean value with error bars representing a 95% confidential interval.