Supplementary Figure 7: Validation of DART-Seq editing adjacent to m⁶A sites.

(a) IGV browser tracks showing C to U editing in APOBEC1-YTH-expressing cells at m⁶A sites in the BSG mRNA (sites with >10% editing are indicated by blue/red (C/U) coloring at the site; BSG is on the positive strand). Editing is not present in cells expressing APOBEC1 alone. Expanded views show Sanger sequencing following RT-PCR from cells expressing APOBEC1-YTH or APOBEC1 alone. C to U editing events (*) near m⁶A sites identified by SCARLET¹⁶ are shown, along with m⁶A abundance at that site as measured by SCARLET. (b) IGV browser tracks show C to U editing in APOBEC1-YTH-expressing cells at m⁶A sites in the ACTB mRNA (sites with >10% editing are indicated by gold/green (C/U) coloring at the site; ACTB is transcribed from the negative strand). Editing is not present in APOBEC1 alone-expressing cells. Expanded views show Sanger sequencing following RT-PCR from cells expressing APOBEC1-YTH or APOBEC1 alone. C to U editing events (*) near m⁶A sites identified by miCLIP⁵ are shown. Results represent a minimum of n = 2 independent experiments.