Supplementary Figure 9: DART-Seq in METTL3-depleted cells.

(a) Motif analysis was performed using regions +/− 4 nt surrounding C to U editing sites identified by DART-Seq in METTL3-depleted (METTL3 KD) cells. Shown is the most highly enriched motif. n = 8,112. P value was calculated using the cumulative binomial distribution. (b) Pie chart showing the distribution of C to U editing events identified by DART-Seq in METTL3 KD cells. n = 2 independent samples. (c) Venn diagram showing the overlap between C to U editing sites called by DART-Seq in wild type (WT) and METTL3 KD HEK293T cells. (d) IGV browser tracks showing read coverage and C to U mutations in wild type and METTL3 KD cells in two representative mRNAs (RPS4X and EEF2; both transcribed from the negative strand). C to U mutations found with at least 10% frequency are indicated by gold/green (C/U) coloring at the mutation site. MeRIP-Seq is shown in blue. C to U mutations in wild type cells are lost in METTL3 KD cells. Images represent 2 independent samples.